In this system, a peptide was immobilized on a commercially available plate bottom. Co-coating procedures yielded 62 and 65% binding of applied 14C-labelled F(ab')2 and F(ab') fragments respectively. A novel peptide-array format system with a photo-cleavage linker for cellular toxicity analysis was constructed.

Application: Fmoc-Photo-Linker is an Fmoc-amino photolabile-linker used in the solid-phase synthesis of peptide amides. Photoimmobilization of anti-PSA F(ab') fragments in the presence of T-BSA revealed exponential binding characteristics indicating stabilizing molecular co-operativity of the BSA constituent. Alternate Names: Fmoc-Photo-Linker is also known as Fmoc-Photolabile Linker. Immuno-responses of photoimmobilized monoclonal anti-AFP antibodies were equivalent to signal intensities obtained with physically adsorbed antibodies. The immunoreagents remained immunologically active after 350 nm irradiation (irradiance 0.7 mW.cm-2 for 20 min). Prior to photo-activation, F(ab')2 or F(ab') fragments derived from anti-(prostate-specific antigen) monoclonal antibodies were mixed and co-coated with the photolinker polymer on to polystyrene microplates. In this and the co-coating procedure described, diazirine-functionalized BSA (T-BSA) served as the multifunctional light-activatable linking agent (photolinker polymer). PhotoLinker provides a whole new level of interactive geotagging and carefully follows the metadata standards, something that other far more expensive photo annotation tools fail to do. Anti-alpha-foetoprotein (anti-AFP) monoclonal antibodies were covalently linked to microplates by layer-coating procedures, which entail antibody photoimmobilization to a photolinker-polymer-precoated surface. is powerful photo annotation and geotagging tool that was launched in 2009. Photolinker-polymer-mediated covalent immobilization of antibodies, F(ab') and F(ab')2 fragments has been achieved by light-dependent coupling procedures.

Photolinker ii